[HTML][HTML] Mutations in the interglobular domain of aggrecan alter matrix metalloproteinase and aggrecanase cleavage patterns: evidence that matrix metalloproteinase …
FA Mercuri, RA Maciewicz, J Tart, K Last… - Journal of Biological …, 2000 - ASBMB
We have expressed G1-G2 mutants with amino acid changes at the DIPEN 341↓ 342
FFGVG and ITEGE 373↓ 374 ARGSV cleavage sites, in order to investigate the relationship
between matrix metalloproteinase (MMP) and aggrecanase activities in the interglobular
domain (IGD) of aggrecan. The mutation DIPEN 341 to DIGSA 341 partially blocked
cleavage by MMP-13 and MMP-8 at the MMP site, while the mutation 342 FFGVG to 342
GTRVG completely blocked cleavage at this site by MMP-1,-2,-3,-7,-8,-9,-13,-14. Each of the …
FFGVG and ITEGE 373↓ 374 ARGSV cleavage sites, in order to investigate the relationship
between matrix metalloproteinase (MMP) and aggrecanase activities in the interglobular
domain (IGD) of aggrecan. The mutation DIPEN 341 to DIGSA 341 partially blocked
cleavage by MMP-13 and MMP-8 at the MMP site, while the mutation 342 FFGVG to 342
GTRVG completely blocked cleavage at this site by MMP-1,-2,-3,-7,-8,-9,-13,-14. Each of the …