Antigen-presenting function and B7 expression of murine sinusoidal endothelial cells and Kupffer cells
AW Lohse, PA Knolle, K Bilo, A Uhrig, C Waldmann… - Gastroenterology, 1996 - Elsevier
AW Lohse, PA Knolle, K Bilo, A Uhrig, C Waldmann, M Ibe, E Schmitt, G Gerken…
Gastroenterology, 1996•ElsevierBACKGROUND & AIMS: Inflammatory liver disease as well as rejection of liver allografts are
thought to be mediated by resident antigen-presenting cells in the liver. At the same time, in
vivo antigen presentation in the liver appears to be a more tolerogenic than systemic antigen
challenge. The aim of this study was to show and characterize the antigen-presenting
capability of sinusoidal endothelial cells and Kupffer cells. METHODS: Purified murine
sinusoidal endothelial cells and Kupffer cells were studied for their ability to serve as …
thought to be mediated by resident antigen-presenting cells in the liver. At the same time, in
vivo antigen presentation in the liver appears to be a more tolerogenic than systemic antigen
challenge. The aim of this study was to show and characterize the antigen-presenting
capability of sinusoidal endothelial cells and Kupffer cells. METHODS: Purified murine
sinusoidal endothelial cells and Kupffer cells were studied for their ability to serve as …
BACKGROUND & AIMS
Inflammatory liver disease as well as rejection of liver allografts are thought to be mediated by resident antigen- presenting cells in the liver. At the same time, in vivo antigen presentation in the liver appears to be a more tolerogenic than systemic antigen challenge. The aim of this study was to show and characterize the antigen-presenting capability of sinusoidal endothelial cells and Kupffer cells.
METHODS
Purified murine sinusoidal endothelial cells and Kupffer cells were studied for their ability to serve as accessory cells and antigen-presenting cells by proliferation assays. They were also studied for their expression of interleukin 1 and the B7 costimulatory molecules by Northern blotting, polymerase chain reaction, and flow cytometry.
RESULTS
Both cell types expressed interleukin 1 messenger RNA and could serve equally well as accessory and antigen-presenting cells. B7-2 messenger RNA and surface expression on sinusoidal endothelial cells and on Kupffer cells was shown. Antibodies to the B7 molecules inhibited antigen presentation. Addition of interleukin 10 as a regulatory cytokine secreted by Kupffer cells was suppressive.
CONCLUSIONS
Sinusoidal endothelial cells carry functional B7-2 molecules and can serve as effective antigen-presenting cells. However, antigen presentation by sinusoidal endothelial cells may be locally down-regulated by interleukin 10. (Gastroenterology 1996 Apr;110(4):1175-81)
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