Identification of the peptide binding motif for HLA-B44, one of the most common HLA-B alleles in the Caucasian population
M DiBrino, KC Parker, DH Margulies, J Shiloach… - Biochemistry, 1995 - ACS Publications
M DiBrino, KC Parker, DH Margulies, J Shiloach, RV Turner, WE Biddison, JE Coligan
Biochemistry, 1995•ACS PublicationsMATERIALS AND METHODS Cells and Antisera. HLA-B44 molecules were isolated from
the human plasma cell line Hmy2. ClR deficient in class I expression (Zemmour et al., 1992)
transfected with the HLA-B* 4403 cDNA. HLA-B44 full-length cDNA was obtained by PCR
amplification (DiBrino et al., 1993) of cDNA made from RNA isolated from the
humanlymphoblastoid B cell line W1B (HLA-A23,-A24,-B44). The cDNA was completely
sequenced to confirm identity to the published sequences for HLA-B* 4403 (Fleischhauer et …
the human plasma cell line Hmy2. ClR deficient in class I expression (Zemmour et al., 1992)
transfected with the HLA-B* 4403 cDNA. HLA-B44 full-length cDNA was obtained by PCR
amplification (DiBrino et al., 1993) of cDNA made from RNA isolated from the
humanlymphoblastoid B cell line W1B (HLA-A23,-A24,-B44). The cDNA was completely
sequenced to confirm identity to the published sequences for HLA-B* 4403 (Fleischhauer et …
MATERIALS AND METHODS
Cells and Antisera. HLA-B44 molecules were isolated from the human plasma cell line Hmy2. ClR deficient in class I expression (Zemmour et al., 1992) transfected with the HLA-B* 4403 cDNA. HLA-B44 full-length cDNA was obtained by PCR amplification (DiBrino et al., 1993) of cDNA made from RNA isolated from the humanlymphoblastoid B cell line W1B (HLA-A23,-A24,-B44). The cDNA was completely sequenced to confirm identity to the published sequences for HLA-B* 4403 (Fleischhauer et al., 1991; Zemmour, 1992, 1993)(previously called HLAB44. 1: new), cloned into the expression vector RSV. neo
ACS Publications