Quantitation of eosinophil and neutrophil infiltration into rat lung by specific assays for eosinophil peroxidase and myeloperoxidase Application in a Brown Norway rat …
T Schneider, AC Issekutz - Journal of immunological methods, 1996 - Elsevier
Conditions for measuring selectively eosinophil peroxidase (EPO) and the neutrophil
myeloperoxidase (MPO) in inflamed rat lung were determined. EPO could be specifically
measured with o-phenylene diamine as chromogen at pH 8.0 in the presence of 3 mM
bromide and MPO with tetramethylbenzidine as chromogen at pH 5.0 in the absence of
bromide but with the EPO inhibitor, resorcinol. Aeroallergen challenge of sensitized Brown
Norway rats with ovalbumin, but not with saline, resulted in a pronounced eosinophilic lung …
myeloperoxidase (MPO) in inflamed rat lung were determined. EPO could be specifically
measured with o-phenylene diamine as chromogen at pH 8.0 in the presence of 3 mM
bromide and MPO with tetramethylbenzidine as chromogen at pH 5.0 in the absence of
bromide but with the EPO inhibitor, resorcinol. Aeroallergen challenge of sensitized Brown
Norway rats with ovalbumin, but not with saline, resulted in a pronounced eosinophilic lung …
