Time-resolved fluorometric assay for natural killer activity using target cells labelled with a fluorescence enhancing ligand
K Blomberg, R Hautala, J Lövgren, VM Mukkala… - Journal of …, 1996 - Elsevier
A time-resolved fluorometric assay for the measurement of natural killer cell activity against
target cells labelled with the acetoxymethyl ester of the fluorescence enhancing ligand 2,
2′: 6′, 2 ″-terpyridine-6, 6 ″-dicarboxylic acid (TDA) is described. The hydrophobic
esterified form of TDA (bis (acetoxymethyl) 2, 2′: 6′, 2 ″-terpyridine-6, 6 ″-dicarboxylate,
BATDA) diffuses readily through the cell membrane of viable cells. BATDA is hydrolysed by
intracellular esterases resulting in accumulation of membrane impermeable TDA inside the …
target cells labelled with the acetoxymethyl ester of the fluorescence enhancing ligand 2,
2′: 6′, 2 ″-terpyridine-6, 6 ″-dicarboxylic acid (TDA) is described. The hydrophobic
esterified form of TDA (bis (acetoxymethyl) 2, 2′: 6′, 2 ″-terpyridine-6, 6 ″-dicarboxylate,
BATDA) diffuses readily through the cell membrane of viable cells. BATDA is hydrolysed by
intracellular esterases resulting in accumulation of membrane impermeable TDA inside the …
