[HTML][HTML] Automated yeast two-hybrid screening for nuclear receptor-interacting proteins
M Albers, H Kranz, I Kober, C Kaiser, M Klink… - Molecular & Cellular …, 2005 - ASBMB
High throughput analysis of protein-protein interactions is an important sector of hypothesis-
generating research. Using an improved and automated version of the yeast two-hybrid
system, we completed a large interaction screening project with a focus on nuclear receptors
and their cofactors. A total of 425 independent yeast two-hybrid cDNA library screens
resulted in 6425 potential interacting protein fragments involved in 1613 different interaction
pairs. We show that simple statistical parameters can be used to narrow down the data set to …
generating research. Using an improved and automated version of the yeast two-hybrid
system, we completed a large interaction screening project with a focus on nuclear receptors
and their cofactors. A total of 425 independent yeast two-hybrid cDNA library screens
resulted in 6425 potential interacting protein fragments involved in 1613 different interaction
pairs. We show that simple statistical parameters can be used to narrow down the data set to …
