[HTML][HTML] JC human polyomavirus is associated to chromosomal instability in peripheral blood lymphocytes of Hodgkin's lymphoma patients and poor clinical outcome
R M'kacher, L Andreoletti, S Flamant, F Milliat… - Annals of oncology, 2010 - Elsevier
R M'kacher, L Andreoletti, S Flamant, F Milliat, T Girinsky, J Dossou, D Violot, E Assaf…
Annals of oncology, 2010•ElsevierBackground B cells are potential sites for latency and reactivation of the human neurotropic
JC polyomavirus (JCV). We investigated JCV and Epstein–Barr virus (EBV) status in
peripheral blood lymphocytes (PBL) from 74 Hodgkin's lymphoma (HL) and 91 B-cell non-
Hodgkin's lymphoma (B-NHL) patients. Patients and methods JCV and EBV DNA were
assessed by PCR, and FISH technique was used to localize viral infection and to estimate
chromosomal instability (rogue cells,'chromosomal aberrations') throughout evolution. The …
JC polyomavirus (JCV). We investigated JCV and Epstein–Barr virus (EBV) status in
peripheral blood lymphocytes (PBL) from 74 Hodgkin's lymphoma (HL) and 91 B-cell non-
Hodgkin's lymphoma (B-NHL) patients. Patients and methods JCV and EBV DNA were
assessed by PCR, and FISH technique was used to localize viral infection and to estimate
chromosomal instability (rogue cells,'chromosomal aberrations') throughout evolution. The …
Background
B cells are potential sites for latency and reactivation of the human neurotropic JC polyomavirus (JCV). We investigated JCV and Epstein–Barr virus (EBV) status in peripheral blood lymphocytes (PBL) from 74 Hodgkin’s lymphoma (HL) and 91 B-cell non-Hodgkin’s lymphoma (B-NHL) patients.
Patients and methods
JCV and EBV DNA were assessed by PCR, and FISH technique was used to localize viral infection and to estimate chromosomal instability (rogue cells, ‘chromosomal aberrations’) throughout evolution. The influence of viral infection and chromosomal instability on freedom from progression (FFP) was investigated in HL patients.
Results
PCR product sequencing of PBL identified JCV in 42 (57%) circulating lymphocytes of HL patients. FISH analysis revealed that the presence of cells with a high JCV genome copy number—associated to the presence of rogue cells and ‘higher frequency of chromosomal aberrations’—increased from 15% before treatment to 52% (P < 10-5) after. The co-activation of JCV and EBV was independent of known prognostic parameters and associated with a shorter FFP (JCV and EBV co-activation P < 0.001, rogue cells P < 0.002).
Conclusion
In HL, JCV activation and chromosomal instability have been identified in PBL and associated with a poorer prognosis, especially in EBV+.
Elsevier