CD14+S100A9+ Monocytic Myeloid-derived Suppressor Cells and Their Clinical Relevance in Non–Small Cell Lung Cancer
PH Feng, KY Lee, YL Chang, YF Chan… - American journal of …, 2012 - atsjournals.org
PH Feng, KY Lee, YL Chang, YF Chan, LW Kuo, TY Lin, FT Chung, CS Kuo, CT Yu, SM Lin…
American journal of respiratory and critical care medicine, 2012•atsjournals.orgRationale: Myeloid-derived suppressor cells (MDSCs) are a heterogeneous family of
myeloid cells that suppress T-cell immunity in tumor-bearing hosts. Their clinical relevance
remains unclear. Objectives: To identify subtypes of myeloid-derived suppressor cells in
patients with non–small cell lung cancer (NSCLC) and their clinical relevance. Methods:
CD11b+ CD14− and CD11b+ CD14+ cells, determined and phenotyped by fluorescence-
activated cell sorter analysis, in the peripheral blood mononuclear cells (PBMCs) of …
myeloid cells that suppress T-cell immunity in tumor-bearing hosts. Their clinical relevance
remains unclear. Objectives: To identify subtypes of myeloid-derived suppressor cells in
patients with non–small cell lung cancer (NSCLC) and their clinical relevance. Methods:
CD11b+ CD14− and CD11b+ CD14+ cells, determined and phenotyped by fluorescence-
activated cell sorter analysis, in the peripheral blood mononuclear cells (PBMCs) of …
Rationale: Myeloid-derived suppressor cells (MDSCs) are a heterogeneous family of myeloid cells that suppress T-cell immunity in tumor-bearing hosts. Their clinical relevance remains unclear.
Objectives: To identify subtypes of myeloid-derived suppressor cells in patients with non–small cell lung cancer (NSCLC) and their clinical relevance.
Methods: CD11b+CD14− and CD11b+CD14+ cells, determined and phenotyped by fluorescence-activated cell sorter analysis, in the peripheral blood mononuclear cells (PBMCs) of treatment-naive patients with advanced NSCLC were correlated with clinical data. T-cell activation in response to CD3/CD28 costimulation was determined by carboxy-fluorescein diacetate succinimidyl ester (CFSE) staining and ELISA analysis of IFN-γ. The percentage of CD11b+CD14+S100A9+ cells in PBMCs was correlated with and tested as a predictor for treatment response in a cohort of patients prospectively receiving first-line cisplatin-based chemotherapy.
Measurements and Main Results: Patients with NSCLC had a significantly higher ratio of CD11b+CD14+ cells than healthy subjects, which was correlated with poor performance status and poor response to chemotherapy. The depletion of these cells in the PBMC reversed the suppression of CD8+ and CD4+ T cells. Isolated CD11b+CD14+ cells suppressed CD8+ T-cell proliferation and IFN-γ production, and the former effect was attenuated by the inducible nitric oxide synthase (iNOS) inhibitor aminoguanidine hydrochloride, arginase inhibitor N-hydroxy-nor-l-arginine (nor-NOHA), and blocking antibodies for IL-4Rα+ and IL-10. CD11b+CD14+ cells were monocyte-like, expressing CD33+, CD15−/low, IL-4Rα+, and S100A9+ and producing iNOS, arginase, and several cytokines. The ratio of S100A9+ cells positively correlated with the suppressive ability of the CD11b+CD14+ cells, was associated with poor response to chemotherapy, and predicted shorter progression-free survival.
Conclusions: CD14+S100A9+ inflammatory monocytes in patients with NSCLC are a distinct subset of MDSCs, which suppress T cells by arginase, iNOS, and the IL-13/IL-4Rα axis. The amount of these inflammatory monocytes is associated with poor response to chemotherapy.
Clinical trial registered with www.clinicaltrials.gov (NCT 01204307).
ATS Journals