[HTML][HTML] Glutamine-dependent NAD+ synthetase: how a two-domain, three-substrate enzyme avoids waste
M Wojcik, HF Seidle, P Bieganowski… - Journal of biological …, 2006 - ASBMB
Glutamine-dependent NAD+ synthetase, Qns1, utilizes a glutamine aminotransferase
domain to supply ammonia for amidation of nicotinic acid adenine dinucleotide (NaAD+) to
NAD+. Earlier characterization of Qns1 suggested that glutamine consumption exceeds
NAD+ production by 40%. To explore whether Qns1 is systematically wasteful or whether
additional features account for this behavior, we performed a careful kinetic and molecular
genetic analysis. In fact, Qns1 possesses remarkable properties to reduce waste. The …
domain to supply ammonia for amidation of nicotinic acid adenine dinucleotide (NaAD+) to
NAD+. Earlier characterization of Qns1 suggested that glutamine consumption exceeds
NAD+ production by 40%. To explore whether Qns1 is systematically wasteful or whether
additional features account for this behavior, we performed a careful kinetic and molecular
genetic analysis. In fact, Qns1 possesses remarkable properties to reduce waste. The …